Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2013 May 27;201(5):693–707. doi: 10.1083/jcb.201302145

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2013 Reczek et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — Cells lacking the Brca1–Ctip interaction are proficient for the MMEJ and SSA pathways of DSB repair. ES cells harboring either the EJ2-GFP (A and B; to measure MMEJ repair) or SA-GFP (C and D; to measure SSA repair) substrate integrated into the Pim1 or Hprt locus, respectively, were transfected with either an I-SceI expression vector or the empty vector. (B) I-SceI expression induced similar levels of GFP-positive cells in cultures of isogenic Ctip^+/− (subclones 1 and 2) and Ctip^S326A/− (subclones 3 and 4) cells with the EJ2-GFP substrate, indicating that the Ctip–S326A mutation does not affect MMEJ. (D) Likewise, I-SceI expression induced comparable levels of GFP-positive cells in cultures of isogenic Ctip^+/− (subclones 1 and 2) and Ctip^S326A/− (subclones 3, 4, and 5) cells with the SA-GFP substrate, indicating that the Ctip–S326A mutation does not affect SSA.