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. 2013 May 27;201(5):653–662. doi: 10.1083/jcb.201210110

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© 2013 Riche et al.

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Figure 5. — An up-regulation of Gα–GPR–LIN-5 leads to NCC overcentration in C. briggsae embryos. (A) Position of the center of the NCC at the end of its rotation is represented as a percentage of cell length. Error bars indicate standard deviation. Statistical differences are shown with a star for comparison to wild-type C. briggsae (for P < 0.05). (B) C. elegans and C. briggsae embryos during NCC rotation stained with antibodies against GPR (in red). Tubulin is in green and DNA in blue. Rectangles show the region where GPR intensity was measured. The anterior pole is to the left. Bar, 10 µm. The histogram shows the mean ratio of GPR levels between anterior and posterior side of the cell for each species. Error bars indicate standard deviation. The star shows statistical difference between species (P < 0.05).