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Coexpression of the trpA1-reporter and Per in pacemaker neurons. Brains from 3- to 7-d-old UAS-nlacZ::GFP/+;trpA1GAL4/+ flies immunostained with anti-β-GAL and anti-Per at ZT23 during LD cycles. The right panels are the merged images. A, LPN. B, LNv. C, LNd. These 3D images were reconstituted from confocal stacks. D, DN1 and 2. E, DN3. The arrowheads indicate colabeling of Per and the trpA1 reporter. Scale bar, 10 μm.
