Table 1.
Genotype | Entrain condition | Entrain temp (°C) | Free run (°C) | n | Rhy (%) | Period (h) | FFT |
---|---|---|---|---|---|---|---|
Wild-type | LD | 25 | 25 | 77 | 95 | 23.93 ± 0.03 | 0.15 ± 0.01 |
trpA1GAL4 | LD | 25 | 25 | 59 | 90 | 23.90 ± 0.04 | 0.17 ± 0.01 |
trpA1GAL4/+ | LD | 25 | 25 | 16 | 100 | 23.83 ± 0.02 | 0.21 ± 0.02 |
trpA11 | LD | 25 | 25 | 32 | 94 | 23.63 ± 0.05 | 0.15 ± 0.01 |
Wild-type | LD | 29 | 29 | 16 | 100 | 23.69 ± 0.06 | 0.15 ± 0.01 |
trpA1GAL4 | LD | 29 | 29 | 16 | 100 | 23.66 ± 0.05 | 0.14 ± 0.01 |
trpA1GAL4/+ | LD | 29 | 29 | 15 | 100 | 23.68 ± 0.03 | 0.14 ± 0.01 |
Wild-type | TC | 25/16 | 25 | 45 | 93 | 23.66 ± 0.08 | 0.15 ± 0.01 |
trpA11 | TC | 25/16 | 25 | 45 | 96 | 23.28 ± 0.04* | 0.13 ± 0.01 |
trpA1GAL4 | TC | 25/16 | 25 | 36 | 83 | 23.11 ± 0.08* | 0.10 ± 0.01 |
trpA1GAL4/trpA11 | TC | 25/16 | 25 | 31 | 100 | 23.36 ± 0.07* | 0.15 ± 0.01 |
UAS-trpA1-A;trpA1GAL4 | TC | 25/16 | 25 | 28 | 96 | 23.64 ± 0.04 | 0.22 ± 0.01 |
UAS-trpA1-A;trpA11 | TC | 25/16 | 25 | 29 | 97 | 23.19 ± 0.07* | 0.08 ± 0.01 |
tim-GAL4/+;trpA11 | TC | 25/16 | 25 | 16 | 81 | 23.32 ± 0.12* | 0.10 ± 0.01 |
tim-GAL4/UAS-trpA1-A; trpA11 | TC | 25/16 | 25 | 16 | 88 | 24.12 ± 0.09 | 0.15 ± 0.02 |
trpA1GAL4/+ | TC | 25/16 | 25 | 16 | 100 | 23.71 ± 0.06 | 0.20 ± 0.02 |
The flies were exposed to either LD or TC using a 12/12 h paradigm. Data are shown as the period and FFT values (±SEM) after the LD or TC cycles during days 2–7 of the free runs.
*p < 0.05, significant differences from wild-type using ANOVA and the Scheffé post hoc tests. Rhy, Rhythmicity.