Table 6.

TAG synthesis from different combinations of substrates by microsomal fractions from developing safflower seeds

Substrates added	nmol [14C]TAG formed/min × mg protein
	12 DAP	17 DAP	22 DAP	27 DAP
[14C]18:1-DAG (PDAT activity)	0.57 ± 0.08	0.66 ± 0.09	0.68 ± 0.04	0.36 ± 0.02
[14C]18:1-DAG + 18:1-CoA (PDAT + DGAT activity)	1.56 ± 0.27	1.16 ± 0.04	1.16 ± 0.12	0.60 ± 0.03
Calculated DGAT activity	0.99	0.50	0.48	0.24
[14C]-18:1DAG + 18:2-CoA (PDAT + DGAT activity)	0.99 ± 0.08	0.92 ± 0.09	0.89 ± 0.04	0.51 ± 0.08
Calculated DGAT activity	0.42	0.26	0.21	0.15
di-6:0-DAG + [14C]18:1-CoA (DGAT activity)	3.28 ± 0.26	2.57 ± 0.08	2.10 ± 0.17	1.67 ± 0.06
di-6:0-DAG + [14C]18:2-CoA (DGAT activity)	2.02 ± 0.13	1.98 ± 0.05	1.69 ± 0.06	0.95 ± 0.1

In experiments with di-6:0-DAG + [¹⁴C]FA-CoA only synthesised di-6:0-TAG is presented; the amount of [¹⁴C]TAG synthesised from endogenous DAG and [¹⁴C]FA-CoA was low (from 2.3 to 14.3 % of synthesised di-6:0-TAG value). Mean ± SD shown (n ≥ 3)

DAP seeds days after pollination used for microsomal membrane preparations