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a. Dot blot with pre-immunised serum obtained from rabbit. Spot 1: 3 μl of proteins extracted from PM102 isolate grown in 0.2% TCE as the sole carbon source. Spot 2: 3 μl of 1 mg/ml BSA. b. Dot blot with immunised serum antibody. Spot A: 3 μl of proteins extracted from PM102 isolate grown in 0.2% TCE as the sole carbon source. Spot B: 3 μl of 1 mg/ml BSA.
