Table 2.
Results of receptor mRNA expression in various sea lamprey tissues by reverse transcriptase-PCR.
| Adult | Parasitic | Larval | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Female | Male | Female | Male | ||||||||||||
| lGnRH-R- | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | 3 | 1 | 2 | |
| Brain | + | + | + | + | + | + | + | + | + | + | + | + | + | − | − |
| Adeno-hypopysis | + | + | − | + | + | − | − | − | − | − | − | − | + | − | − |
| Gonad | − | − | − | + | − | − | − | − | + | − | + | + | − | # | + |
| Heart | # | # | + | # | + | + | − | + | − | − | + | − | − | − | − |
| Muscle | + | − | + | + | + | + | − | + | − | + | + | − | # | − | − |
| Liver | + | # | + | # | − | + | + | + | + | − | + | − | − | − | − |
| Eye | + | + | + | + | + | + | − | + | + | + | + | + | + | − | − |
| Intestine | + | + | + | + | + | − | + | + | − | − | + | − | − | − | − |
| Kidney | − | − | − | + | − | − | − | − | − | + | − | − | + | − | − |
| Skin | + | − | − | − | − | − | NA | NA | NA | NA | NA | NA | − | − | − |
| Thyroid | + | + | # | + | − | # | NA | NA | NA | NA | NA | NA | # | − | − |
| Gill | NA | NA | NA | NA | NA | NA | − | − | − | + | + | + | NA | NA | NA |
Reactions were done on cDNAs made from pooled total RNA from individually extracted tissues in the adult and parasitic phase lampreys. In larval samples tissues were pooled first and then extracted. The following samples were pooled as follows: 4 per adults, 3 per parasitic phase lampreys, and 20 per larval lampreys.
+, message detected; −, message not detected; #, message detected only at 5× cDNA (100 ng/ reaction) concentration relative to original assay; NA, sample not available.