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. 2013 May 28;7:100. doi: 10.3389/fncir.2013.00100

Figure 5.

Figure 5

The M602A insertion traps ADAMTS3. (A) Structure of the ADAMTS3 locus and the integration site of M602A. M602A is inserted in the 3rd intron of ADAMTS3. The long horizontal bar and vertical bars denote the genomic DNA and the exons of ADAMTS3, respectively. ADAMTS3 consists of multiple functional domains: pro, pro domain; PD, metalloprotease domain; TSP1, thrombospondin type 1 motif; spacer, spacer region [according to Porter et al. (2005)]. (B,C) Expression of ADAMTS3 at 24 hpf (B) and 48 hpf (C), detected by in situ hybridization. (D–F) Expression of ADAMTS3 and EGFP detected by double fluorescent in situ hybridization. A confocal section of the spinal cord of the M602A;UAS:GFP embryo at 24 hpf was shown. The dotted lines demarcate the dorsal and ventral limit of the spinal cord. The scale bars indicate 20 kbp (top) and 50 amino acids (bottom) in (A), and 20 μm in (F).