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. 2013 Apr 5;41(10):5303–5320. doi: 10.1093/nar/gkt207

Figure 4.

Figure 4.

The effect of B. subtilis DnaC on DnaG primase activity as a function of ssDNA concentration. (A) Representative chromatograms showing the RNA primers produced by B. subtilis DnaG with 2, 4 or 8 µM d(CTA)-containing ssDNA template in the absence or presence of DnaC and DnaEBs. Concentration of the protein(s) and ssDNA template used in the priming assays are indicated in the right panels, with the respective chromatogram on the left. The 6-mer, 10-mer and 16-mer RNA polymers are denoted and were based on the elution of RNA standards. Priming reactions contained 0.8 mM each NTP. (B) Quantification and comparison of RNA primers synthesized by B. subtilis DnaG in the absence or presence of DnaC and DnaEBs with 2, 4 or 8 µM of the d(CTA)-containing ssDNA template. Priming reactions were carried out as described in A with various combinations of DnaG (G) and DnaC (C).