Figure 9.
The effects of DnaG primase and DnaC helicase on the native DnaEBs polymerase activity. Representative alkaline agarose gels showing DnaEBs primer extension time courses in the presence or absence of effector proteins, DnaG, DnaC, DnaC/DnaI, DnaC–DnaG and DnaC/DnaI–DnaG, as indicated. 10 nM DnaEBs was premixed with different proteins: 30 nM DnaG, 60 nM monomeric DnaC and 60 nM monomeric DnaI, as appropriate. Reactions were carried out as described in ‘Materials and Methods’ section. Lanes marked 0 represent the radioactive template before protein addition; lanes G, C, I, CI and CG represent primer extension control reactions (16 min) in the presence of DnaG, DnaC, DnaI, DnaC/DnaI and DnaC–DnaG, respectively. Molecular weight markers are labelled appropriately.