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. 2013 Apr 5;41(10):5210–5222. doi: 10.1093/nar/gkt223

Figure 2.

Figure 2.

S7 interacts with GADD45α. (A) The purified GST–GADD45α or GST proteins and 293T whole-cell lysate with high levels of S7 expression were incubated together, and then GST-pull-down assay was performed to examine the in vitro interaction between GADD45α and S7. (B) 293T cells were either transfected with the expression plasmid encoding FLAG–GADD45α or in combination with GFP–S7 construct or eFGPN1 vector. Cell lysate was immunoprecipitated with anti-GFP antibody, and then the immunoprecipitants were probed with anti-GFP and anti-FLAG antibodies. (C) HepG2 cells were transfected with FLAG–GADD45α construct or its control vector. Cell lysate was immunoprecipitated with anti-FLAG antibody, and the immunoprecipitants were probed with anti-FLAG, anti-GADD45α and anti-S7 antibodies. (D) HepG2 cells were treated with arsenite (20 µM) for 12 h, and then cell lysate was immunoprecipitated with anti-GADD45α antibody or rabbit IgG. The immunoprecipitants were probed with anti-GADD45α and anti-S7 antibodies.