Skip to main content
. 2013 Apr 12;41(10):5273–5289. doi: 10.1093/nar/gkt224

Figure 5.

Figure 5.

Effects of variants of the proximal HSP70A promoter on histone modifications at transgenic HSP70A and RBCS2 promoters. (A) Optimal spacing and intact HSE2 and TATA-box in the proximal HSP70A promoter are essential for mediating increased H3 acetylation at transgenic promoters. Quantification of precipitated chromatin fragments and significance tests were done as described in Figure 4A using data on nucleosome occupancy shown in Supplementary Figures S3D and S3E. (B) Optimal spacing and intact HSE2 and TATA-box in the proximal HSP70A promoter are essential for mediating increased H4 acetylation at transgenic promoters. ChIP was done using antibodies against acetylated lysines 5, 8, 12 and 16 of histone H4. Normalization was done as described in Figure 4A. (C) Optimal spacing but not intact HSE2/TATA-box are required for reducing H3K9 monomethylation levels at transgenic promoters. ChIP was done using antibodies against monomethylated lysine 9 at histone H3 (H3K9me1). Normalization was done as described in Figure 4F.