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. 2013 Apr 10;41(10):5368–5381. doi: 10.1093/nar/gkt225

Figure 4.

Figure 4.

Reprogramming serves as a survival response in xpa-1. (A) Body length of WT and xpa-1 nematodes fed with empty vector control (con), sti-1, spc-1 and sma-1 RNAi measured when WT nematodes on control food reached the L4/adult stage. Results are presented as medians (line) or means (square) inside the boxes showing the 25–75 percentile range with whiskers presenting SD. The lengths of 150 (spc-1 and sma-1 RNAi) or 90 (control and sti-1) were measured for each condition. (B) The measured levels of oxidized DNA bases in DNA isolated from WT and xpa-1 mutant nematodes. The DNA lesions were measured using GC/MS/MS and the results represent average lesion levels ± standard deviation from 5 to 7 independent biological replicates. Statistical analysis was performed using one-way ANOVA with Newman–Keuls post-test. *P < 0.05, **P < 0.01. (C) The measured levels of oxidized DNA nucleosides in DNA isolated from WT and xpa-1 mutant nematodes. The DNA lesions were measured using LC/MS/MS and the results represent average lesion levels ± standard deviation from 5 to 7 independent biological replicates. Statistical analysis was performed using one-way ANOVA with Newman–Keuls post-test. *P < 0.05, **P < 0.01.