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. 2013 Jun;194(2):389–401. doi: 10.1534/genetics.113.149740

Figure 2.

Figure 2

The Rad53 FHA domains required a T105-x-x-E-L motif in the Dbf4 N terminus for interaction. (A and B) An alanine scan of all Dbf4 threonines within the minimal Rad53-binding region (residues 100–227) using two-hybrid assays against FHA1 and FHA2, respectively. (C) Summary of Dbf4 mutants within residues 100–114 for their effect on the interaction of FHA1 and FHA2 domains. Growth assays are shown in Figure S2. (D) Two-hybrid assays showing the effect of additional T105 and T171 mutations on the Dbf4–FHA1 interaction.