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. 2013 Jun;194(2):389–401. doi: 10.1534/genetics.113.149740

Figure 5.

Figure 5

dbf4-NΔ109 was synthetically lethal with rad53-R70A, rad53-K227A, and rad53-G653E. (A) Schematic diagram of Rad53 showing the mutations studied within the FHA1 and FHA2 domains. (B) The rad53-G653E and rad53-N655A mutants did not interact with Dbf4 in yeast two-hybrid assays. (C) Western blot of the full-length Rad53-GAD fusion used for two-hybrid analyses and the corresponding FHA domain mutants. The Ponceau S-stained blot is shown below as a loading control. (D) Representative tetrads from diploid strains of genotype DBF4/dbf4-NΔ109 RAD53/rad53-R70A and DBF4/dbf4-NΔ109 RAD53/rad53-K227A were sporulated and dissected onto YPD plates. Recombinant genotypes are indicated.