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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Gastroenterology. 2012 Apr 14;143(2):469–480. doi: 10.1053/j.gastro.2012.04.011

Figure 1.

Figure 1

Characterization of the LSL-Mist1myc transgenic line. (A) Standard H&E, amylase (AMY), and Cx32 immunofluorescence depicts adult Mist1KO acini showing similarities to embryonic cells, in contrast to adult, fully developed WT acinar cells. Arrows, Cx32 gap junctions. E, embryonic day. (B) Schematic of the Mist1Cre-ER genomic locus and LSL-Mist1myc transgene. (C) Genomic PCR and immunoblots reveal the TM-restricted recombination and expression of the LSL-Mist1myc transgene. (D) Upon TM, only acinar cells express nuclear MIST1myc.protein (arrows). D, duct. (E) Time-course of MIST1myc expression after TM treatment. Scale bars: (A) 10 µm; (D) 50 µm.