Figure 4.
Chromatin immunoprecipitation assays confirm MIST1 interaction with target genes. (A and B) Pancreas chromatin from 3 WT mice was processed for ChIP assays using anti-IgG (control) or anti-MIST1. In most cases, MIST1 binding was enriched significantly in the anti-Mist1 groups. (C) Notably, no enrichment was observed with control genes (β-tubulin) or at alternative sites within target genes (eg, Rnd2 +2.9 kb). In addition, Nox4 showed no MIST1 binding at the predicted MIST1 binding site. The positions of the amplicon regions with respect to each gene are indicated below the graphs.