Figure 5.

Induced MIST1^myc rescues the Mist1^KO phenotype in adult acinar cells. (A and B) H&E and carboxypeptidase (CPA) immunofluorescence reveals the organization of zymogen granules within individual acini units of WT, Mist1^KO, and Mist1^KO/LSL-Mist1^myc pancreata 1, 3, 7, and 21 days after TM. (C) Transmission electron microscopy views of individual acinar cells showing the mislocalized zymogen granules (arrows) in Mist1^KO samples. (D and E) Analysis of lumen size in WT, Mist1^KO, and Mist1^KO/LSL-Mist1^myc acini by zonula occludens 1 (ZO-1) immunofluorescence and transmission electron microscopy. White outlines, individual acini; red outlines, individual lumens. DAPI, 4′,6-diamidino-2-phenylindole. Scale bars: (A, B, and D) 10 µm; (C and E) 2 µm.