Figure 6.
Induced MIST1myc rescues the actin cytoskeleton and cell size defects of Mist1KO acinar cells. (A) Anti–β-actin immunofluorescence showing restoration of the terminal (white arrows) and cortical (yellow arrows) actin webs after TM-induced MIST1myc expression. (B) E-cadherin immunofluorescence and quantification of acinar and islet cell areas over the TM time course. (C and D) LC3B immunofluorescence and TEM reveal that Mist1KO acinar cells show high levels of autophagic vacuoles, whereas induced MIST1myc expression reduces their presence toWT levels. (C) LC3B-positive cells (arrows); (D) individual autophagic vesicles (arrows). Scale bars: (A, B, and C) 10 µm; (D) 0.5 µm. DAPI, 4′,6-diamidino-2-phenylindole; E-cad, E-cadherin.