Figure 3.

Scribble expression in stably transfected HEK293s cells expressing CD74 under the control of tetracycline-inducible promoter. (A) Western blot analysis showing the same Scribble abundance in the fully denatured and unfolded samples extracted from control and tetracycline-induced cells. (B) The same cell cultures as in A show increased Scribble staining in the CD74-overexpresing cells in FACS assays despite the fact that the same anti-Scribble primary antibody is used. The filled-in gray histogram is from secondary antibody-only control. (C) The same cell cultures as in A and B show increased Scribble staining by confocal microscopy. The same primary antibody is used.