Figure 5.

Overexpression of CD74 induces translocation of a subpopulation of Scribble molecules from the basolateral side to the apical side of the cells. GFP-Scribble was transiently transfected in TetO-CD74 HEK293s cells and the expression of CD74 was induced with tetracycline. After 24 hours of induction, the cells were fixed and stained with anti-CD74 antibody and analyzed by laser scanning confocal microscopy. The image is presented as “Slice View,” in which the three orthogonal planes XY (the midsection of the stack), YZ, and XZ are formed by slicing the stack as indicated by the crossed orange lines. The white arrows point to the apical side of the cells where GFP-Scribble can be seen only in the cells overexpressing CD74.