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. Author manuscript; available in PMC: 2014 Mar 19.

Published in final edited form as: Biochemistry. 2013 Mar 8;52(11):1858–1873. doi: 10.1021/bi3015696

a. The dependence of the fluorescence anisotropy of the DnaT N-terminal core domain upon the total domain monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0), recorded at different temperature: 20°C, 25°C, 30°C, and 35°C. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). b. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 295 nm, λ_em = 347 nm) in buffer C (pH 7.0), containing 0.1 mM EDTA and no magnesium, recorded at different temperature: 20°C, 25°C, 30°C, and 35°C. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model (accompanying paper, eqs. 3 - 7). c. The dependences of the trimerization constant, K_T, upon the reciprocal of the temperature (Kelvin) (van’t Hoff plot), in the presence and absence of magnesium, respectively. The solid lines are linear least-squares fits to the van’t Hoff equation (details in text).

Inline graphic — a. The dependence of the fluorescence anisotropy of the DnaT N-terminal core domain upon the total domain monomer concentration (λ_ex = 296 nm, λ_em = 347 nm) in buffer C (pH 7.0), recorded at different temperature: 20°C, 25°C, 30°C, and 35°C. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model described by eqs. 3 - 7 (accompanying paper). b. The dependence of the DnaT fluorescence anisotropy upon the total DnaT monomer concentration (λ_ex = 295 nm, λ_em = 347 nm) in buffer C (pH 7.0), containing 0.1 mM EDTA and no magnesium, recorded at different temperature: 20°C, 25°C, 30°C, and 35°C. The solid lines are the nonlinear least-squares fits of the titration curves, using the trimer model (accompanying paper, eqs. 3 - 7). c. The dependences of the trimerization constant, K_T, upon the reciprocal of the temperature (Kelvin) (van’t Hoff plot), in the presence and absence of magnesium, respectively. The solid lines are linear least-squares fits to the van’t Hoff equation (details in text).