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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Prostate. 2011 Dec 27;72(12):1306–1316. doi: 10.1002/pros.22479

Fig. 5. IL-6 induced phosphorylation of JAK2 in LNCaP-C3 but not in LNCaP-S17 cells.

Fig. 5

A. Expression of IL-6R, gp130, and JAK family in LNCaP-C3 (C3) and LNCaP-S17 (S17) cells in regular culture medium without any treatment. B. Phosphorylation of JAK2 in LNCaP-C3 (C3) and LNCaP-S17 (S17) cells treated without or with 50 ng/ml IL-6 in serum-free medium for the indicated time. C. LNCaP-C3 (C3) and LNCaP-S17 (S17) cells were treated without or with 50 ng/ml IL-6 in serum-free medium for 5 min. Equal amounts of total proteins were precipitated with anti-p-JAK2 antibodies and protein A-sepharose beads. Western blot analysis was performed as described under “Materials and Methods”.