Table 1.
SFE operational conditions tested.
| Assay | T (°C) | Time (min) | CO2 flow (g min−1) | Pressure (bar) | Modifier | Modifier (%) | Modifier flow (mL min−1) | Fractions obtained |
|---|---|---|---|---|---|---|---|---|
| None | None | None | A100, A120, A140, A160, A200, A250, A300 | |||||
| 1 | 40 | 30 | —* | 100, 120, 140, 160, 200, 250, 300 | Ethanol | —* | 0.1 | B100, B120, B140, B160, B200, B250, B300 |
| —* | 0.2 | C100, C120, C140, C160, C200, C250, C300 | ||||||
|
| ||||||||
| 2 | 40 | 30 | 3 | 100, 120, 140, 160, 200, 250, 300 | Ethanol | 3 6 |
0.1 0.2 |
D100, D120, D140, D160, D200, D250, D300
E100, E120, E140, E160, E200, E250, E300 |
|
| ||||||||
| 3 | 40 | 30 | 3 | 140 | Ethanol Methanol |
0, 0.5, 1, 1.5, 2, 2.5, 3 | 0.0, 0.02, 0.03, 0.05, 0.06, 0.08, 0.1 | F0, F0.5, F1, F1.5, F2, F2.5, F3
G0, G0.5, G1, G1.5, G2, G2.5, G3 |
*In pressure mode, neither CO2 flow nor modifier percentage were controlled.