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AFS cells cultured on soft surfaces increase migration rates of other AFS cells. Crystal violet-stained AFS cells that migrated through Transwell inserts towards AFS cells cultured on (A) 2 kPa substrates, (B) plastic, or (C) Chang's media only. Average number of observed migrated (D) AFS cells per field of view. Scale bar: 50 μm. Significance: ** p<0.01.
