. Author manuscript; available in PMC: 2013 May 28.

Published in final edited form as: J Chromatogr A. 2009 Nov 10;1217(25):4135–4143. doi: 10.1016/j.chroma.2009.11.006

Table 2.

Doseresponse data in human cells and rat tissue. (A) dG-ABP adduct content and absolute standard deviation from RT-4 human bladder carcinoma cells that were treated with 4-ABP dissolved in DMSO. Cells were cultured in triplicate, pooled, and analyzed in triplicate. (B) Adduct quantity and absolute standard deviation from bladder tissue DNA extracted from twelve 2-month old F/344/NHsd rats that were administered 4-ABP prepared in DMSO. DNA from replicate doses was pooled before analysis and injections were completed in triplicate

Concentration of 4-ABP in DMSO (μM)	Analyte/internal standard by peak area	fmol dG-ABP on column	Number of adducts detected in 10⁸ nucleosides
(A) 4-ABP dosed human bladder RT-4 cells
0	Not detected	Not detected	Not detected
0.5	0.065±0.005	0.2±0.3	4±8
5.0	0.14±0.03	0.9±0.5	20±10
50	4.0±0.8	40±8	1100±200
Concentration of 4-ABP in DMSO IP injection (mg 4-ABP/kg body weight)	Analyte/internal standard by peak area	fmol dG-ABP on column	Number of adducts detected in 10⁸ nucleosides

(B) 4-ABP dosed F/344/NHsd rats
0	Not detected	Not detected	Not detected
25	0.34±0.09	3±1	80±30
100	0.49±0.08	4.4±0.9	120±20
250	0.5±0.1	4±1	120±30