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Protein expression of HIF-1α, OPN, and ON on day 28 at 1%, 2%, and 20% O2 with and without the HIF-1 inhibitor in 3-D culture. A representative gel is shown in (A). Results of three independent studies are quantified in (B) HIF-1α, (C) OPN, and (D) ON. The values have been normalized to β-actin and represent the mean±standard deviation. When a statistically significant difference (p<0.05) is observed between 20% O2 in comparison to 1% and 2% O2, it is marked (*).
