FIG. 4.

Proliferation and cytokine secretion of purified T cells in coculture with ECMp. Purified CFSE-labeled T cells were cocultured without stimulation (unstim), or with low-dose anti-CD3 (stim), in uncoated plates or in plates coated with 50 μg/mL porcine (porc), bovine (bov), or human (hum) collagens or elastins for 5 days before supernatants were collected and cells were labeled with antibodies and analyzed by flow cytometry in a CFSE-based proliferation assay. The percentage of proliferated T cells (a) was determined by gating on all viable cells followed by gating on the CD3+ population and measuring divided cells based on dilution of the CFSE signal in a histogram. An ELISA was used to determine the amount of (b) IFN-γ and (c) IL-10 present in the supernatants. All data are given as mean±SEM from independent experiments (n=4). Asterisks indicate a significant difference compared to the a-CD3-stimulated control (*p<0.05 or **p<0.01 or ***p<0.001).