Figure 3. .
Pro-inflammatory activity of γδ T cells in immunized B6 mice is decreased after ATRA-treatment. Assessment of the proinflammatory effect of γδ T cells in vitro. Responder T cells were isolated from the spleen and draining lymph nodes of IRBP1-20/CFA-immunized TCR-δ−/− mice at 13 days after immunization. Then, T responder cells (1 × 106/well) were stimulated in 24-well plates for 48 hours with an optimal dose of immunizing peptide (10 μg/mL) under Th17 polarizing conditions. The control group contained responder αβ T cells alone, while the test groups aloes contained 2% (2 × 104/well) of γδ T cells isolated from ATRA-treated or untreated mice. After 5 days of in vitro stimulation, the activated T cells were separated and intracellularly stained with PE-conjugated anti–IFN-γ antibodies and FITC-conjugated anti–IL-17 antibodies, followed by FACS analysis. A representative study of more than 5 repeats is shown.