Figure 5. .
Splenic APCs from ATRA-treated mice are functionally less effective in promoting activation of IL-17+ autoreactive T cells. (A) Responder T cells (1 × 106/well) from immunized B6 mice were stimulated in vitro for 48 hours in 24-well plates with IRBP1-20 in the presence of splenic APCs from ATRA-treated or nontreated mice for 5 days, then activated T cells were stained for expression of IL-17 and IFN-γ, αβTCR, or γδTCR. (B) ELISA assay. The culture supernatant from splenic T cells from immunized mice that were left untreated or treated with ATRA were tested for IL-17 production after 48 hours of stimulation with the immunizing peptide IRBP1-20 in the presence of splenic APCs from either ATRA-treated or untreated mice. (C) Recipient mice injected with ATRA generate adequate numbers of the CD25+ DC subset. Splenic cells from ATRA-treated and nontreated immunized mice were stained for expression of CD11c and CD25.