Figure 7. .

RA antagonists prevent the effect of ATRA on the differentiation of BMDCs. BMDCs were treated with LPS (A), LPS plus ATRA (B), or LPS plus ATRA plus two RA antagonists (BMS 195614 binding to RARα and CD2665 binding to RARβ/γ), both at a final concentration of 1 μM (C). The washed DCs were cultured with CD3-enriched splenic T cells from immunized B6 mice, then the activated T cells were separated and stained with the indicated antibodies. (D) The 48-hour culture supernatants from the experiments in Figure 5A were assayed for IL-17 using ELISA kits.