| Reagent | 50 μl Reaction | 20 μl Reaction |
| Water | 27 μl | 10.8 μl |
| 5X PCR reaction buffer | 10 μl | 4 μl |
| MgCl2 | 4 μl | 1.6 μl |
| dNTP (10 mM) | 1 μl | 0.4 μl |
| Primer mix (5 uM each) | 2 μl | 0.8 μl |
| Taq (Promega Hotstart) | 1 μl | 0.4 μl |
| ChIP DNA | 5 μl | 2 μl |
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