FIG. 2.
The GAG mimetic [OTR4120] potentiates the hMSC in vitro commitment to the osteogenic lineage. hMSC were expanded in a control (culture medium [CM]) or osteogenic (OS) medium supplemented or not with [OTR4120] during 21 days. (A) Alizarin Red staining of the calcium deposits. Scale bar: 1 mm. (B) Flow cytometry analysis of alkaline phosphatase (ALP) expression. (C, D) Quantitative real-time polymerase chain reaction analysis of the expression of bone morphogenic protein-2 (BMP-2), osteocalcin (OC), and osteopontin (OP) after 7 days (C) and 14 days (D) of treatment. Expression levels are reported to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and presented as 2−ΔCt. Values are means±SD of triplicate per condition and per experiment, from three independent experiments (n=9; *p<0.05; **p<0.01). Color images available online at www.liebertpub.com/tea