Figure 2.
Compounds 10 and 16 were evaluated in a biofilm assay where they showed a dose-dependent pattern with an estimated IC50 around 30–40 μM for compound 10 and 65–75 μM for 16. The corresponding carboxylic acid substituted pilicide 2 displayed a 45 % inhibition at 400 μM. The figure shows the average inhibition of duplicate runs and the standard deviations are shown in error bars.