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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Biochemistry. 2013 Apr 24;52(18):3157–3170. doi: 10.1021/bi400231s

Figure 4. ‘Off-pathway’ primosome assembly results in the formation of metastable aggregates.

Figure 4

(a) In this experiment gp41 (0.3 JM), GTP (6 JM) and gp61 (50 nM) were premixed and injected into the sample chamber. These mixing conditions resulted in scattered excitation light from aggregated structures in the illuminated sample area. (b) Samples prepared using the two-step assembly pathway showed no evidence of aggregation throughout all the measurements, indicating that the two-step assembly pathway (gp41+GTP and then gp61) avoids the formation of metastable aggregates. The image is split into donor (left) and acceptor (right) channels. A donor molecule and its paired acceptor molecule are separated by 255 pixels on the CCD camera (represented as three paired boxes in Fig. 4b).