Figure 4.
Podocytes activate T cells by crosspresentation. Crosspresentation and activation of the ovalbumin-specific H2-Kb–restricted T cell hybridoma B3Z. DCs, BMMs, or PCLs were incubated with different concentrations of ovalbumin for 18 or 44 hours. After antigen uptake, serial dilutions of the cells were cocultured in 96-well plates with 50,000 B3Z T cells (APC/T cell ratios from 1:1 to 0.01:1). After 16 hours, the T cell activation was analyzed as described. (A and B) LPS-activated DCs incubated with ovalbumin for 18 or 44 hours in the presence of 0.1 µg/ml LPS. (C and D) BMMs incubated with ovalbumin for 18 or 44 hours. (E) Unstimulated PCLs incubated with ovalbumin for 44 hours. (F) PCLs stimulated with TNF-α (20 ng/ml) and incubated with ovalbumin for 44 hours. Individual values present the mean of three individual experiments ± SD.