Figure 4.

SLC41A1 localizes predominantly to distal convoluted tubules. (A) Immunohistochemistry was performed on human paraffin-embedded sections using rabbit anti-SLC41A1 antibodies. Signal was seen primarily in the DCTs. G, glomerulus. Arrowhead shows macula densa. (B) Rat paraffin-embedded sections were immunostained with antibodies against SLC41A1 (red) and Claudin 16 (green). DAPI (blue) was used to stain cell nuclei. (C) RT-PCR was performed on human nephron segments with primers for SLC41A1 and actin-β (ACTB) as loading control. CCD, cortical collecting duct; NTC, no template control; PCT, proximal convoluted tubule; TAL, thick ascending limb.