Table 2.
Human and mouse antibodies utilized for immunofluorescence and flow cytometry analyses
| Antibody | Dilution | Manufacturer |
|---|---|---|
|
Immunofluorescence | ||
|
Human | ||
| CD31 |
1:50 |
Sigma |
| vWF |
1:50 |
Sigma |
| GLUT-1 |
1:100 |
Millipore |
| e-NOS |
1:100 |
Millipore |
| UEA-1 |
1:20 |
Sigma |
| hNAg |
1:20 |
Chemicon |
| Draq 5 |
1:800 |
Alexis |
| VE-cadherin |
1:250 |
Santa Cruz |
| Claudin-5 |
1:1000 |
Invitrogen |
| β-catenin |
1:250 |
Abcam |
|
Mouse | ||
| CD31 |
1:100 |
Millipore |
| vWF |
1:50 |
Santa Cruz Biotechnology |
| GLUT-1 |
1:100 |
Millipore |
| e-NOS |
1:100 |
Millipore |
|
Flow cytometry | ||
|
Human | ||
| CD31-PE (AC128) |
1:40 |
Miltenyi |
| CD105-FITC ( SN6) |
1:40 |
Serotec |
| CD146-PE ( F4-35H7) |
1:20 |
Biocytex |
| UEA-1-FITC |
1:50 |
Sigma |
|
Mouse | ||
| CD31-PE (MEC13.3) |
1:20 |
BD Biosciences |
| CD34-FITC (RAM34) |
1:20 |
BD Biosciences |
| CD146-FITC (P1H12) |
1:20 |
Chemicon |
| Tie-2-PE (1E11DH) | 1:50 | Millipore |