Table 3.

RT-PCR primer sequences and expected product size

Primers	Sequences (5’-3’)	Product size (bp)	Annealing T (°C)
Human
CD31-f	GCT GTT GGT GGA AGG AGT GC	645	49
CD31-r	GAA GTT GGC TGG AGG TGC TC
vWF-f	CAC TGA CAC CTG AGT GAG AC	696	60
vWF-r	GTT CGT CCT GGA AGG ATC GG
Tie-2-f	GGC CGC TAC CTA CTA ATGA AG	550	50
Tie-2-r	CGT GAT TGA CAC TGG ACA TAA
P-gp-f	GCA AAG CTG GAG AGA TCC TCA CCA	305	60
P-gp-r	CAA CAT TTT CAT TTC AAC AAC TCC TGC
GAPDH-f	CGG AGT CAA CGG ATT TGG TCG TAT	307	58
GAPDH-r	AGC CTT CTC CAT GGT GGT GAA GAC
Mouse
CD31-f	GTC ATG GCC ATG GTC GAG TA	260	55
CD31-r	CTC CTC GGC GAT CTT GCT GAA
vWF-f	TGT TCA TCA AAT GGT GGG CAG C	270	62
vWF-r	ACA GAC GCC ATC TCC AGA TTC A
Tie-2-f	GGA CAG TGC TCC AAC CAA ATG	154	60
Tie-2-r	GAC GGA AAT GTT GAA AGG C
P-gp-f	TGC TTA TGG ATC CC	435	55
P-gp-r	TTG GTG AGG ATC TCT CCG GCT
GAPDH-f	GTC GGT GTG AAC GGA TTT G	280	55
GAPDH-r	TAG ACT CCA CGA CAT ACT CAG CA