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. Author manuscript; available in PMC: 2013 May 28.
Published in final edited form as: J Neurochem. 2008 Oct 24;107(5):1314–1324. doi: 10.1111/j.1471-4159.2008.05691.x

Fig. 3. Immunocytochemical detection of phosphorylated GRK7 in HEK-293 cells.

Fig. 3

Cells were incubated in the absence (A, B, C) or presence (D, E, F) of 50 μM FSK and 1 mM IBMX for 30 min. Cells were stained simultaneously with anti-Flag (A, D) and anti-pGRK7 (B, E) antibodies at 1:1,500 and 1:100, respectively. Panel C represents the merging of images A and B. Panel F represents the merging of images D and E.