Figure 2. Evaluation of wild-type and mutant KCC3^ΔCterm, KCC3^mPro functions in Xenopus oocyte flux assays.

(A) Wild-type KCC3 and mutant KCC3^ΔCterm, KCC3^mPro transit to the plasma membrane in Xenopus Laevis oocytes. Immunofluorescence staining of KCC3 using a specific antibody shows all generated forms at the plasma membrane of the injected oocytes. Control oocytes were injected with water instead of the wild-type and mutant KCC3 cRNAs. (B) Directed mutagenesis of the proline motif leads to impaired function of KCC3 in Xenopus Laevis oocyte flux assay. ⁸⁶Rb⁺ flux of wild-type and mutant KCC3 is measured under hypotonic conditions in presence or absence of KCCs inhibitor, furosemide.