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A) The conformation of cloned pCR-pLDH containing the pLDH gene by digestion with EcoRI. M, Molecular size marker; lane 1, non-inserted clone; lane 2, pLDH gene inserted clone (pVpLDH). B) Confirmation of pLDH gene in Eschelichia coli DH5α by restriction enzyme digestion with EcoRI and HindIII. M, Molecular size marker; lane 1, Undigested Plasmid; lane 2, BamHI digested Plasmid; lane 3, EcoRI and HindIII digested plasmid.
