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. 2013 May 28;8(5):e64671. doi: 10.1371/journal.pone.0064671

Figure 4. Analysis of the peptidoglycan digestion products obtained with phage lytic proteins.

Figure 4

MS/MS analysis, in positive mode, of the main peaks obtained in the S. aureus SA113 peptidoglycan digestion with (A) HydH5SH3b and (B) HydH5Lyso. A) MS/MS of the m/z = 1180.7 ion yielded two main fragmentation products. m/z = 959.5 ion corresponds to the product of removal of the GlcNAc, and m/z = 774.5 ion is in agreement with the peptidoglycan structure MurNAc-(L-Ala-D-iGln-L-Lys-(NH2-Gly3). B) MS/MS of the m/z = 1123.6 ion yielded two main daughter ions. m/z = 717.5 ion corresponds to the peptidoglycan structure MurNAc-(L-Ala-D-iGln-L-Lys-(NH2-Gly2) and m/z = 902.5 corresponds to the structure MurNAc-(L-Ala-D-iGln-L-Lys-(NH2-Gly4)-D-Ala-COOH).

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