Figure 3. Treg-depletion augmented disease severity and decreased survival in CLP-treated mice.

DEREG and C57BL/6 mice pre-treated with DT (1 µg in 100 µl PBS i.v. on days −2 and −1) were subjected to CLP. Survival was monitored for 5 days (A) and disease severity was scored for 3 days (B) by an observer blinded for the group assignment. Data from four independent experiments with similar results are summarized (each experiment involved 8–11 mice/group). Median and interquartile range of the disease severity score are shown in panel B; ** p<0.01.