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. 2013 Apr 17;6:411–417. doi: 10.2147/OTT.S43744

Figure 2.

Figure 2

FCM analysis for apoptosis after treatment by annexin V-FITC and PI staining on MCF-7 cells. After treatment with different doses of 5-Aza-CdR for 48 hours, apoptosis induction was observed. Apoptotic cells were differentiated from viable or necrotic ones by combined application of annexin V-FITC and PI. This assay was performed in triplicate. (A) Blank control group; (B) 0.4 μmol/L 5-Aza-CdR group; (C) 1.6 μmol/L 5-Aza-CdR group; (D) 6.4 μmol/L 5-Aza-CdR group; (E) 25.6 μmol/L 5-Aza-CdR group; (F) 102.4 μmol/L 5-Aza-CdR group. (G) comparison of all groups.

Notes: *P < 0.05; **P < 0.01 versus control group.

Abbreviations: 5-Aza-CdR, 5-aza-2′-deoxycytidine; FCM, flow cytometry; FITC, fluorescein isothiocyanate; PI, propidium iodide.