Table 4.
Hypergeometric distribution analysis results to determine enrichment of autism candidate genes from AutDB and AutismKB
| Autism gene database | Total number of genes in database | Total promoter regions on array | Total IP RORA-binding promoter regions | RORA-binding autism genes (overlap) | Pvalue |
|---|---|---|---|---|---|
| AutDB (SFARI gene) |
328 |
25,500 |
2,544 |
49 |
0.0028 |
| AutismKB (syndromic + non-syndromic) |
3,050 |
25,500 |
2,544 |
426 |
<0.001 |
| AutDB + AutismKB | 3,158 | 25,500 | 2,544 | 438 | <0.001 |
For the hypergeometric distribution analyses, the total number of general markers (that is, the population) is the number of genes (25,500) whose promoter regions are represented on the array, and the total number of selected markers is the number of unique genes (2,544) identified as targets of RORA by the ChIP-on-chip analysis. The total interesting markers are the number of autism candidate genes in the respective or combined databases, and the number of selected interesting markers are the genes within our dataset that overlap with those in either or the combined databases.