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. 1973 Dec;52(6):633–637. doi: 10.1104/pp.52.6.633

Amino Acid Uptake by Pea Leaf Fragments

Specificity, Energy Sources, and Mechanism 1

Yuk-Ngai Stephen Cheung a, Park S Nobel a
PMCID: PMC366561  PMID: 16658620

Abstract

Amino acid uptake into leaf fragments of Pisum sativum depended on metabolism. Glycine uptake was optimal at 30 C and could be supported by respiration and by photosynthesis. Based on studies with an electron flow cofactor, inhibitors, and uncouplers, the energy source for glycine uptake was apparently ATP.

The energy-dependent transport of glycine was mediated by a carrier that had a broad specificity for neutral and positively charged l-amino acids. It readily translocated 15 such l-amino acids into the cells, but had a very low affinity for l-aspartate, l-glutamate, d-amino acids, and α-aminoisobutyrate. The Ki for competitive inhibition of glycine uptake by another amino acid was equal to the Km for the uptake of that competing species.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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