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. 2013 May 28;8(5):e65513. doi: 10.1371/journal.pone.0065513

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© 2013 Chen et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Percentage of methylation at 9 CpG sites in the PRSS8 promoter determined by bisulfite sequencing of genomic DNA extracted from UROtsa cells chronically treated with CSE, UROCSE-p32 (32^nd passage) and from untreated cells, UROtsa-p32 (32^nd passage). Percentage of methylation was calculated by the number of methylated cytosines at each CpG site observed in the total number of independent clones sequenced for each BS DNA amplicon, n = 60 for both UROtsa-p32 and UROCSE-p32.