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. 2013 May 1;11:107. doi: 10.1186/1479-5876-11-107

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Copyright © 2013 Yang et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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CSF tracer localization after intracisternal and lumbar intrathecal infusion. (A-F) Localization of FITC-conjugated dextran (FITC-d500, MW 500kD) and Texas Red-conjugated dextran (TR-d3, MW 3 kD) 30 min after intracisternal infusion. Large molecular weight FITC-d500 remained restricted to perivascular spaces (arrows) surrounding penetrating arteries (A-B) and extending to the level of the terminal capillary beds (C-F, arrows). Small molecular weight TR-d3 moved quickly into the brain interstitium and was taken up by subpopulations of neurons (arrowheads). (G-L) 120 min after lumbar intrathecal infusion of tracers, large molecular weight FITC-d500 accumulated in perivascular spaces (arrows), but not as uniformly as observed after intracisternal infusion. Small molecular weight TR-d3 moved readily throughout the brain parenchyma. Inset (L) depicts IB4 staining and background green and red fluorescence in tissue from animals not injected with CSF tracer. GFAP: glial fibrillary acidic protein (astrocytic marker); IB4: isolectin B4 (vascular endothelial marker).