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. Author manuscript; available in PMC: 2014 May 1.
Published in final edited form as: Cell Calcium. 2013 Mar 13;53(0):315–326. doi: 10.1016/j.ceca.2013.02.001

FIGURE 3. Characteristics and activation of 4 wk old WT and Trpc1−/− BMMCs.

FIGURE 3

A: Toluidine blue staining revealed a difference in morphology and granularity between 4 wk old WT (left) and Trpc1−/− (right) BMMCs. B: Reduced expression of FcεRI and KIT receptor in Trpc1−/− as revealed by flow cytometry. C,D: β-hexosaminidase release assay in 4 wk old BMMCs prepared from WT and Trpc1−/− mice show reduced degranulation in Trpc1−/− BMMCs. Anti-DNP-IgE-sensitized cells were treated with increasing concentrations of antigen (C) or Ag and SCF (30 ng/ml) (D) for 30 min. The data are presented as the mean ± SEM (n=3) separate experiments conducted in duplicate (*p<0.05, **p<0.01; two tailed Student’s t-test).